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- 约翰年代. Toll Summer Research Fellow
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Exploring Cellular Pathways
凯特林 马里诺
2019届毕业生九州娱乐官网
- Beta Beta Beta
EXTRACURRICULAR ACTIVITIES
- Women's Volleyball
SUPPORT FROM DONOR PROGRAM
A John Toll Fellow who conducted neuroscientific research on campus, 凯特林马里诺
’19 used a zebrafish model to test memory after stroke.
A zebrafish egg looks like nothing more than a tiny bubble of carbonated water, but
this translucent organism contains a world of hope for the scientific research community
investigating how to treat stroke in human patients.
凯特林马里诺 ’19, a dual major in biology and psychology with a concentration in
behavioral neuroscience, is part of that community. In the lab of her faculty adviser,
Assistant Professor of 生物学 Jim Windelborn, she carefully collected fertilized
eggs from the bottom of a narrow fish tank she and Windelborn built specifically for
this summer research project meant to establish that, in fish, hypoxia affects memory.
Working under a microscope and using a microinjector, 马里诺 inserteda single strand
of DNA into the embryo and returned the eggs to the tank, where they then grew into
adult zebrafish whose cells have been genetically altered to express a specific protein.
As part of the experiment, she and Windelborn introduced their subjects to hypoxia—mimicking
the oxygen deficiency that stroke victims experience when a blocked artery disrupts
the flow of blood and oxygen to the brain. Then they ran a series of behavioral assays
to look at memory and how it is affected by ischemic stroke. Using a novel object
recognition test, 马里诺 introduced an object into the tank—in this case, toy Smurfs—right
before the fish have hypoxia. A day later, in fully oxygenated water, the fish were
introduced to that object again, along with a new object.
“The idea is, we’re seeing how much time they spend with the novel object versus what
is considered a familiar object,” 凯特林 explained. “We’re also looking at how brain
tissue is affected 24 hours post-hypoxia.”
It’s part of a long-term project that can help identify different protein targets
and drugs that can bind with those proteins, Windelborn says.
“We are generally bad at treating strokes. There isn’t a whole lot to do after the
fact,” says Windelborn, who studied the cellular and molecular bases of neurological
traumas and neurological diseases as a doctoral student at University of Wisconsin,
麦迪逊. “What’s important is to identify new targets.
Right now, we’re pretty far away from the clinic, but we’re finding that zebrafish
offer a good model for identifying possible therapeutic targets.”
For 马里诺, this was her second summer in the Windelborn lab working on a zebrafish
model for ischemic stroke that will also form the basis of her senior capstone project.
She intends to pursue a doctoral degree in neuroscience and a career as a research
科学家.
“I like the kind of research we’re doing now where you have a big thing you are looking
at—in our case, behavior—and we’re really targeting something cellular, so that’s
where my interest comes from,” says 马里诺. “You can see more of a cause and effect.
I like cellular pathways—the idea of linking biology to behavior.”
凯特林 is now a PhD student in the neuroscience training program at the University
of Wisconsin, 麦迪逊. She is supported fully by a training grant that allows her
to explore research in multiple
laboratories before committing to a lab for her dissertation research. The labs through
which 凯特林 has chosen to rotate have a focus on the mechanisms of the brain’s recovery
from traumas such as seizures and strokes.